3OQ5

Crystal structure of the 3-MBT domain from human L3MBTL1 in complex with p53K382me1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-08-12
Detector	NOIR-1
Wavelength(s)	1.0
Spacegroup name	P 32
Unit cell lengths	109.080, 109.080, 90.050
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.651 - 2.501
R-factor	0.2066
Rwork	0.205
R-free	0.23440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2rje
RMSD bond length	0.006
RMSD bond angle	0.781
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.6.1_357))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.651	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.133	0.538
Number of reflections	41472
<I/σ(I)>	7.2	2.1
Completeness [%]	100.0	100
Redundancy	5.4	5.28

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.8	291	0.1 M sodium acetate pH 4.8, 4% PEG 4000 and 0.1 M sodium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 291K