3OPY

Crystal structure of Pichia pastoris phosphofructokinase in the T-state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-03-13
Detector	RAYONIX MX225HE
Wavelength(s)	0.918
Spacegroup name	P 1 21 1
Unit cell lengths	161.658, 188.303, 231.560
Unit cell angles	90.00, 92.85, 90.00

Refinement procedure

Resolution	29.800 - 3.050
R-factor	0.2019
Rwork	0.200
R-free	0.23180
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.240
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	BUSTER (2.8.0)

Data quality characteristics

	Overall
Low resolution limit [Å]	29.800
High resolution limit [Å]	3.050
Number of reflections	262877
Completeness [%]	53.2
Redundancy	97.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	4.6	292	1 microliter of protein + 1 microliter of reservoir using streak seading, protein: 1mg/mL PFK, 10mM imidazol, 5mM mercaptoethanol, 10% glycerol, 10mM ATP, pH 7.0; reservoir: 0.7M ammonia sulfate, 0.1M sodium citrate pH 4.6, VAPOR DIFFUSION, temperature 292K