3OPX
Crystal structure of pyrimidine 5 -nucleotidase SDT1 from Saccharomyces cerevisiae complexed with uridine 5'-monophosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-03-26 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.9194 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 41.198, 78.037, 82.789 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.201 - 1.700 |
R-factor | 0.2141 |
Rwork | 0.212 |
R-free | 0.25090 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3onn |
RMSD bond length | 0.007 |
RMSD bond angle | 1.092 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX ((phenix.refine: 1.6_289)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 1.730 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.051 | 0.347 |
Number of reflections | 30137 | |
<I/σ(I)> | 39.6 | 5.4 |
Completeness [%] | 99.9 | 100 |
Redundancy | 7.3 | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 287 | 30% PEG4000, 0.1M Tris-HCl buffer, pH 8.5, 0.2M sodium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 287.0K |