3OOL
I-SceI complexed with C/G+4 DNA substrate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-08 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | .979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 129.184, 49.938, 63.360 |
| Unit cell angles | 90.00, 107.54, 90.00 |
Refinement procedure
| Resolution | 30.790 - 2.300 |
| R-factor | 0.2496 |
| Rwork | 0.246 |
| R-free | 0.29400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1r7m |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.146 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
| Rmerge | 0.051 | 0.041 | 0.174 |
| Number of reflections | 22091 | ||
| <I/σ(I)> | 18 | ||
| Completeness [%] | 84.1 | 97 | 86.9 |
| Redundancy | 3.4 | 3.6 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.7 | 277.15 | PEG400,CaCl2,NaOAc,DTT, pH 4.7, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K |
