3OML

Structure of full-length peroxisomal multifunctional enzyme type 2 from Drosophila melanogaster

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2008-02-25
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9310
Spacegroup name	P 43 21 2
Unit cell lengths	114.480, 114.480, 89.110
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.100 - 2.150
R-factor	0.23686
Rwork	0.234
R-free	0.28542
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 1PN4 AND 1GZ6
RMSD bond length	0.011
RMSD bond angle	1.202
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.100	2.210
High resolution limit [Å]	2.150	2.150
Number of reflections	31008
Completeness [%]	99.7	99.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	294	0.1 M Tris, 1.0 M NaCl, 20 % (w/v) PEG 5000 MME, 5 mM NAD+ , VAPOR DIFFUSION, SITTING DROP, temperature 294K, pH 8.0