3OMF
Crystal structure of a histidine triad family protein from Entamoeba histolytica, bound to AMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E+ SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-08-19 |
Detector | RIGAKU SATURN 944+ |
Wavelength(s) | 1.541780 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 53.580, 60.630, 67.880 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.560 - 1.800 |
R-factor | 0.148 |
Rwork | 0.146 |
R-free | 0.19100 |
Structure solution method | MR, MR |
Starting model (for MR) | 3oj7 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.516 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 34.560 | 1.850 | |
High resolution limit [Å] | 1.800 | 8.050 | 1.800 |
Rmerge | 0.023 | 0.018 | 0.045 |
Number of reflections | 9684 | 135 | 626 |
<I/σ(I)> | 52.96 | 94.7 | 21.4 |
Completeness [%] | 91.6 | 94.4 | 80.3 |
Redundancy | 5.8 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 289 | Internal tracking number 217183B6. JCSG screen condition B6: 40% v/v ethanol, 5% w/v PEG 1000, phosphate-citrate buffer pH 4.2, EnhiA.01296.a.A1 PS00632 at 83.2 mg/ml, 10 mM AMP, VAPOR DIFFUSION, SITTING DROP, temperature 289K |