3OL9
Poliovirus polymerase elongation complex with 3'-deoxy-CTP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-06-13 |
Detector | NOIR-1 |
Wavelength(s) | 1.00 |
Spacegroup name | P 1 |
Unit cell lengths | 60.499, 60.555, 192.353 |
Unit cell angles | 83.69, 83.67, 77.45 |
Refinement procedure
Resolution | 41.376 - 2.250 |
R-factor | 0.2038 |
Rwork | 0.203 |
R-free | 0.24880 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ra6 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.064 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.160 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.066 | 0.473 |
Number of reflections | 140748 | |
<I/σ(I)> | 5.3 | 1.4 |
Completeness [%] | 85.3 | |
Redundancy | 1.9 | 1.75 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 283 | 95 mM trisodium citrate, pH 5.6-5.9, 6.5-7.0% (v/v) isopropanol, 16.5-18.0% (w/v) PEG4000, 5% (v/v) glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 283K |