3OJ3
Crystal structure of the A20 ZnF4 and ubiquitin complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-29 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.830, 170.030, 66.239 |
| Unit cell angles | 90.00, 90.10, 90.00 |
Refinement procedure
| Resolution | 43.064 - 2.500 |
| R-factor | 0.2041 |
| Rwork | 0.203 |
| R-free | 0.22580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | : 1UBQ PDB ENTRY: 2FID |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.035 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 31541 | |
| <I/σ(I)> | 15.3 | 3.5 |
| Completeness [%] | 96.2 | 98.2 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 292 | 0.1 M MES pH 6.5 and 30% PEG 4000, VAPOR DIFFUSION, temperature 292K |
