3OIJ
Crystal structure of Saccharomyces Cerevisiae Nep1/Emg1 bound to S-adenosylhomocysteine and 2 molecules of cognate RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-11-07 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.00931 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 83.377, 91.860, 96.931 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.886 - 3.000 |
R-factor | 0.1901 |
Rwork | 0.186 |
R-free | 0.25970 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2v3j |
RMSD bond length | 0.008 |
RMSD bond angle | 1.290 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | PHENIX (1.6_289) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 3.000 |
High resolution limit [Å] | 2.900 | 6.230 | 2.900 |
Rmerge | 0.078 | 0.049 | 0.361 |
Number of reflections | 16553 | ||
<I/σ(I)> | 21.2 | ||
Completeness [%] | 97.0 | 99.8 | 76.5 |
Redundancy | 6.3 | 6.7 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 0.05M MES, 15mM MgS04, 7% PEG 4000, 20% glycerol, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |