3OID
Crystal Structure of Enoyl-ACP Reductases III (FabL) from B. subtilis (complex with NADP and TCL)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-14 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.376, 110.683, 85.169 |
| Unit cell angles | 90.00, 91.82, 90.00 |
Refinement procedure
| Resolution | 36.010 - 1.800 |
| R-factor | 0.15086 |
| Rwork | 0.149 |
| R-free | 0.18061 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3oic |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.138 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.057 | 0.128 |
| Total number of observations | 1861429 | |
| Number of reflections | 100171 | |
| <I/σ(I)> | 34.7 | 8.95 |
| Completeness [%] | 99.2 | 94.9 |
| Redundancy | 3.1 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 1.6M sodium citrate , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
