3OG3
Crystal structure of an artificial thermostable (BA)8-barrel protein from identical half barrels
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OXFORD DIFFRACTION ENHANCE ULTRA |
| Temperature [K] | 123 |
| Detector technology | CCD |
| Collection date | 2009-12-18 |
| Detector | OXFORD RUBY CCD |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 31.374, 82.086, 74.511 |
| Unit cell angles | 90.00, 90.11, 90.00 |
Refinement procedure
| Resolution | 14.650 - 2.080 |
| R-factor | 0.15194 |
| Rwork | 0.150 |
| R-free | 0.19591 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1THF (Amino acids 99-219) dimer |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.006 |
| Data reduction software | CrysalisPro |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 14.700 | 2.140 |
| High resolution limit [Å] | 2.080 | 2.040 |
| Number of reflections | 11304 | |
| <I/σ(I)> | 18.58 | 3.8 |
| Completeness [%] | 99.6 | 96.8 |
| Redundancy | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.2 M ammonium sulfate, 18% PEG 4000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
