3OEV
Structure of yeast 20S open-gate proteasome with Compound 25
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Detector technology | CCD |
| Detector | MAR CCD 165 mm |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 136.067, 299.946, 145.621 |
| Unit cell angles | 90.00, 113.17, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.850 |
| R-factor | 0.2203 |
| Rwork | 0.220 |
| R-free | 0.25690 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.158 |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.950 |
| High resolution limit [Å] | 2.850 | 6.140 | 2.850 |
| Rmerge | 0.100 | 0.039 | 0.467 |
| Number of reflections | 247646 | ||
| <I/σ(I)> | 8.5 | ||
| Completeness [%] | 98.7 | 98.8 | 91 |
| Redundancy | 3.6 | 3.5 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 100 mM MES, pH 7.0, 40 mM MgOAc, 15% 2-methyl-2,4-pentanediol (MPD), 10 mM EDTA, vapor diffusion, hanging drop, temperature 293K |
