3ODO
Crystal Structure of the DH/PH Domains of p115-RhoGEF
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-17 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97921 |
| Spacegroup name | P 41 |
| Unit cell lengths | 106.049, 106.049, 125.973 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.000 - 2.900 |
| R-factor | 0.223 |
| Rwork | 0.221 |
| R-free | 0.27200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1x86 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.833 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.950 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.060 | 0.750 |
| Number of reflections | 30747 | |
| <I/σ(I)> | 31.5 | 2.3 |
| Completeness [%] | 99.3 | 99.9 |
| Redundancy | 4.5 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 2.6-2.8M sodium formate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
