3OCH
Chemically Self-assembled Antibody Nanorings (CSANs): Design and Characterization of an Anti-CD3 IgM Biomimetic
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2004-02-21 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 61 |
| Unit cell lengths | 91.714, 91.714, 73.130 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 23.200 - 1.790 |
| R-factor | 0.20517 |
| Rwork | 0.203 |
| R-free | 0.24174 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rh3 |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.218 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | X-PLOR |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.890 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Rmerge | 0.094 | 0.180 |
| Number of reflections | 32398 | |
| <I/σ(I)> | 10 | 1.8 |
| Completeness [%] | 98.7 | 98.3 |
| Redundancy | 7.7 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 273 | 14-24% PEG 6K, 200 mM MnCl2, 18-24 mM KPO4, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 273K |
