3OBB
Crystal structure of a possible 3-hydroxyisobutyrate Dehydrogenase from pseudomonas aeruginosa pao1
Replaces: 3CUMReplaces: 2H78Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97921 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 92.611, 92.611, 124.860 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.320 - 2.200 |
| R-factor | 0.19908 |
| Rwork | 0.197 |
| R-free | 0.24774 |
| Structure solution method | SAD |
| Starting model (for MR) | none |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.649 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.320 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.116 | 0.747 |
| Number of reflections | 16608 | |
| <I/σ(I)> | 32.6 | 3.56 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 10.5 | 10.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 293 | 4M AMMONIUM ACETATE, 0.1M SODIUM ACETATE, PH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
