3O8O
Structure of phosphofructokinase from Saccharomyces cerevisiae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1997-04-28 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 1.1044 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 180.046, 186.205, 236.507 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.000 - 2.900 |
R-factor | 0.2586 |
Rwork | 0.259 |
R-free | 0.30920 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1pfk |
RMSD bond length | 0.011 |
RMSD bond angle | 1.619 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 2.950 |
High resolution limit [Å] | 2.900 | 2.900 |
Number of reflections | 172818 | |
<I/σ(I)> | 15.13 | 1.75 |
Completeness [%] | 98.5 | 98 |
Redundancy | 4 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 6-10% PEG4000, 0.2 mM sodium acetate, 0.1 M MES buffer pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |