3O89
Crystal Structure of Sperm Whale Myoglobin G65T
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Detector technology | CCD |
| Collection date | 2009-06-13 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 6 |
| Unit cell lengths | 90.439, 90.439, 45.247 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.990 - 1.100 |
| R-factor | 0.1727 |
| Rwork | 0.172 |
| R-free | 0.18290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.034 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.070 |
| High resolution limit [Å] | 1.050 | 2.850 | 1.050 |
| Rmerge | 0.049 | 0.035 | 0.556 |
| Number of reflections | 87374 | ||
| <I/σ(I)> | 17.7 | ||
| Completeness [%] | 88.8 | 97.7 | 14.6 |
| Redundancy | 3.7 | 5.9 | 1.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 8.5 | 293 | 2.6M AS, 50mM Tris 8.5, 1mM EDTA, EVAPORATION, temperature 293K |
