3O7U
Crystal structure of Cytosine Deaminase from Escherichia Coli complexed with zinc and phosphono-cytosine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-10-22 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97915 |
Spacegroup name | H 3 2 |
Unit cell lengths | 145.212, 145.212, 200.609 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.208 - 1.708 |
R-factor | 0.1741 |
Rwork | 0.173 |
R-free | 0.19310 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1k6w |
RMSD bond length | 0.007 |
RMSD bond angle | 1.017 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | BALBES |
Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 39.208 |
High resolution limit [Å] | 1.708 |
Number of reflections | 86951 |
Completeness [%] | 98.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 35% Pentaerythritol Propoxylate, 0.05M Hepes, 0.2M Potassium Chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |