3O7Q
Crystal structure of a Major Facilitator Superfamily (MFS) transporter, FucP, in the outward conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 119.460, 119.460, 98.360 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.643 - 3.143 |
| R-factor | 0.2183 |
| Rwork | 0.215 |
| R-free | 0.27290 |
| Structure solution method | SAD |
| RMSD bond length | 0.047 |
| RMSD bond angle | 1.352 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.3_473)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.643 | 3.250 |
| High resolution limit [Å] | 3.143 | 3.140 |
| Rmerge | 0.077 | 0.871 |
| Number of reflections | 14221 | |
| <I/σ(I)> | 21.12 | |
| Completeness [%] | 98.3 | 99.4 |
| Redundancy | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 291 | 34% PEG400, 0.1M cacodylate, 0.1M MgCl2, 0.05M NaF, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
