3O7L
Crystal Structure of phospholamban (1-19):PKA C-subunit:AMP-PNP:Mg2+ complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2009-01-01 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.00 |
Spacegroup name | P 61 |
Unit cell lengths | 92.193, 92.193, 192.186 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.086 - 2.800 |
R-factor | 0.216 |
Rwork | 0.216 |
R-free | 0.28700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1atp |
RMSD bond length | 0.006 |
RMSD bond angle | 0.951 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASES |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.880 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.084 | |
Number of reflections | 26095 | |
<I/σ(I)> | 38.8 | 5.1 |
Completeness [%] | 99.5 | 100 |
Redundancy | 10.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | The complex was obtained by combining a 1:10:10:10 molar ratio mixture of PKA-C (7 mg/ml), PLN1-19, MgCl2, and AMP-PNP in 20 mM sodium acetate (pH 6.5), 180 mM KCl, and 5 mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |