3O6W
Crystal structure of monomeric KlHxk1 in crystal form VIII (open state)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-29 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.91841 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.540, 93.590, 114.290 |
| Unit cell angles | 90.00, 102.42, 90.00 |
Refinement procedure
| Resolution | 29.120 - 1.480 |
| R-factor | 0.1673 |
| Rwork | 0.167 |
| R-free | 0.18391 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3o08 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.409 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.520 |
| High resolution limit [Å] | 1.480 | 1.480 |
| Number of reflections | 171952 | |
| <I/σ(I)> | 21.7 | 2.5 |
| Completeness [%] | 99.6 | 99.8 |
| Redundancy | 3.6 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.6 | 292 | 1microL reservoir + 1microL protein, reservoir: 2.4M di-ammonia phosphate, 0.1M Ches pH 9.6, protein: 10mg/ml Klhxk1, 10mM Tris pH 7.4, 1mM EDTA, 1mM DTT, 0.5mM PMSF, 1mM xylose, 1mM ATP, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
