3O5Z
Crystal structure of the SH3 domain from p85beta subunit of phosphoinositide 3-kinase (PI3K)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, Hamburg |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-09-03 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.8123 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.010, 57.790, 62.970 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.370 - 2.010 |
| R-factor | 0.2156 |
| Rwork | 0.212 |
| R-free | 0.25890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.884 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.400 | 2.120 |
| High resolution limit [Å] | 2.010 | 2.010 |
| Rmerge | 0.049 | 0.535 |
| Number of reflections | 11641 | |
| <I/σ(I)> | 20.3 | 3.8 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.1 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 285 | 30% MPD, 0.1M sodium cacodylate, 0.05M calcium acetate, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 285K |
