3O47

Crystal structure of ARFGAP1-ARF1 fusion protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-05-11
Detector	RAYONIX MX-300
Wavelength(s)	0.97949
Spacegroup name	I 21 21 21
Unit cell lengths	79.298, 129.161, 157.685
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.650 - 2.800
R-factor	0.2072
Rwork	0.206
R-free	0.24330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	pdb entries 1HUR 3dwd
RMSD bond length	0.009
RMSD bond angle	1.080
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	BUSTER-TNT (BUSTER 2.8.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	2.900
High resolution limit [Å]	2.800	6.030	2.800
Rmerge	0.116	0.038	0.990
Number of reflections	20250
<I/σ(I)>	8.3
Completeness [%]	99.9	99.7	99.8
Redundancy	4.9	4.7	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291	20% PEG-3350, 0.2M trilithium sulfate, pH not applicable, vapor diffusion, temperature 291K