3O41
Crystal Structure of 101F Fab Bound to 15-mer Peptide Epitope
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2009-04-03 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.8266 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 79.899, 92.985, 141.221 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.079 - 1.950 |
| R-factor | 0.1795 |
| Rwork | 0.177 |
| R-free | 0.21700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dqd 1iqw 2j88 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.941 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.479 | |
| Number of reflections | 73963 | |
| <I/σ(I)> | 2 | |
| Completeness [%] | 95.7 | 77.4 |
| Redundancy | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 20.5% (w/v) PEG 4000, 0.2 M lithium sulfate monohydrate, 0.1 M Tris-HCl pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
