3O2P
A Dual E3 Mechanism for Rub1 Ligation to Cdc53: Dcn1(P)-Cdc53(WHB)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2009-12-05 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97918 |
Spacegroup name | P 61 |
Unit cell lengths | 57.157, 57.157, 177.725 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 33.065 - 2.233 |
R-factor | 0.1745 |
Rwork | 0.169 |
R-free | 0.22640 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3bq3 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.953 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.230 |
Number of reflections | 15730 |
Completeness [%] | 98.0 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 27% PEG 3000, 0.1M Tris, 0.2M Lithium Sulfate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |