3O2P

A Dual E3 Mechanism for Rub1 Ligation to Cdc53: Dcn1(P)-Cdc53(WHB)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	77
Detector technology	CCD
Collection date	2009-12-05
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97918
Spacegroup name	P 61
Unit cell lengths	57.157, 57.157, 177.725
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.065 - 2.233
R-factor	0.1745
Rwork	0.169
R-free	0.22640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3bq3
RMSD bond length	0.007
RMSD bond angle	0.953
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.5_2))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.230
Number of reflections	15730
Completeness [%]	98.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	277	27% PEG 3000, 0.1M Tris, 0.2M Lithium Sulfate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K