3O2J

Structure of the GluA2 NTD-dimer interface mutant, N54A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9763
Spacegroup name	P 21 21 21
Unit cell lengths	82.330, 92.580, 102.480
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	51.240 - 1.950
R-factor	0.1933
Rwork	0.191
R-free	0.24660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3hsy
RMSD bond length	0.007
RMSD bond angle	0.999
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.6.1_357)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	68.698	51.240	2.060
High resolution limit [Å]	1.950	6.170	1.950
Rmerge		0.031	0.729
Rmeas		0.033	0.788
Rpim		0.013	0.297
Total number of observations		13502	58129
Number of reflections	57819
<I/σ(I)>	10.6	25.8	2.7
Completeness [%]	100.0	99.8	100
Redundancy	6.9	6.7	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	291	18% PEG 3350, 100mM sodium citrate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K