3O28
Ligand-binding domain of GluA2 (flip) ionotropic glutamate receptor in complex with an allosteric modulator
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-04-15 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.95375 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 65.131, 88.719, 47.620 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 52.490 - 2.000 |
R-factor | 0.18426 |
Rwork | 0.182 |
R-free | 0.23383 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.019 |
RMSD bond angle | 1.836 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 52.490 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.132 | 0.474 |
Number of reflections | 18586 | |
<I/σ(I)> | 11.2 | 3.7 |
Completeness [%] | 99.9 | 100 |
Redundancy | 3.6 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 277 | 16% PEG 8000, 100mM Sodium Cacodylate pH 4.5, 50mM Lithium Sulphate, VAPOR DIFFUSION, HANGING DROP, temperature 277K |