3O23

Human unphosphorylated IGF1-R Kinase domain in complex with an hydantoin inhibitor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	AREA DETECTOR
Collection date	2002-04-01
Spacegroup name	P 1 21 1
Unit cell lengths	49.768, 48.755, 70.730
Unit cell angles	90.00, 98.74, 90.00

Refinement procedure

Resolution	30.000 - 2.100
Rwork	0.238
R-free	0.27800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1jqh
Phasing software	AMoRE
Refinement software	CNX (2000)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.100
Number of reflections	18424
Completeness [%]	91.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	40% PEG400, 50mM Mes, 100mM NaCl, 10% glycerol, 5mM DTT, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K