3NZH

Crystal structure of anti-emmprin antibody 5F6 FAB

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	95
Detector technology	CCD
Collection date	2008-08-08
Detector	RIGAKU SATURN 944
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	96.530, 129.740, 42.390
Unit cell angles	90.00, 109.52, 90.00

Refinement procedure

Resolution	15.000 - 2.000
R-factor	0.19737
Rwork	0.195
R-free	0.24274
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB entries 1NCA 1pz5
RMSD bond length	0.011
RMSD bond angle	1.407
Data reduction software	d*TREK (9.6L)
Data scaling software	d*TREK (9.6L)
Phasing software	PHASER
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.237	0.419
Number of reflections	30870
<I/σ(I)>	4.8	1.8
Completeness [%]	93.4	82
Redundancy	6.1	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	20% PEG 8K, 0.1 M HEPES PH 7.5 CRYO CONDITIONS: 20% PEG 8K, 0.1 M HEPES PH 7.5, 20% GLYCEROL, VAPOR DIFFUSION, SITTING DROP, temperature 293K