3NZ8
Crystal structure of the HIV-2 neutralizing Fab fragment 7C8
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I711 |
Synchrotron site | MAX II |
Beamline | I711 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-09-14 |
Detector | MAR CCD 130 mm |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 100.071, 100.071, 196.763 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.800 - 2.700 |
R-factor | 0.229 |
Rwork | 0.226 |
R-free | 0.27300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1i9j |
RMSD bond length | 0.007 |
RMSD bond angle | 1.065 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 44.600 |
High resolution limit [Å] | 2.700 |
Rmerge | 0.070 |
Number of reflections | 32045 |
<I/σ(I)> | 21.1 |
Completeness [%] | 100.0 |
Redundancy | 7.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 293 | 50 mM ammonium sulphate, 25% (w/v) PEG 8000 and 2.5% (v/v) PEG 400, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |