3NZ8
Crystal structure of the HIV-2 neutralizing Fab fragment 7C8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-09-14 |
| Detector | MAR CCD 130 mm |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 100.071, 100.071, 196.763 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.800 - 2.700 |
| R-factor | 0.229 |
| Rwork | 0.226 |
| R-free | 0.27300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1i9j |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.065 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 44.600 |
| High resolution limit [Å] | 2.700 |
| Rmerge | 0.070 |
| Number of reflections | 32045 |
| <I/σ(I)> | 21.1 |
| Completeness [%] | 100.0 |
| Redundancy | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 293 | 50 mM ammonium sulphate, 25% (w/v) PEG 8000 and 2.5% (v/v) PEG 400, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
