3NYJ

Crystal Structure Analysis of APP E2 domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2010-04-12
Detector	ADSC QUANTUM 315
Wavelength(s)	1.14
Spacegroup name	P 21 21 2
Unit cell lengths	114.979, 40.149, 58.376
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 3.200
Rwork	0.297
R-free	0.37970
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	3.310
High resolution limit [Å]	3.200	3.200
Rmerge	0.081	0.368
Number of reflections	4888
<I/σ(I)>	14
Completeness [%]	100.0	100
Redundancy	13

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.6	298	20% PEG 4000, 20% isopropanol, 0.1M Na Citrate, pH 5.6, vapor diffusion, temperature 298K