3NSP

Crystal structure of tetrameric RXRalpha-LBD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Detector technology	IMAGE PLATE
Collection date	2009-06-03
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 21 21 2
Unit cell lengths	114.828, 100.271, 47.157
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.900
R-factor	0.22627
Rwork	0.222
R-free	0.30755
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1g1u
RMSD bond length	0.013
RMSD bond angle	1.527
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	29.720
High resolution limit [Å]	2.900
Number of reflections	10888

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	100mM Tris, pH6.5, 10% PEG 4000, 5% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 277K