3NR2
Crystal structure of Caspase-6 zymogen
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR-H |
| Temperature [K] | 291 |
| Detector technology | CCD |
| Collection date | 2007-12-08 |
| Detector | BRUKER SMART 6000 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 127.983, 127.983, 167.912 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.898 - 2.900 |
| R-factor | 0.1847 |
| Rwork | 0.179 |
| R-free | 0.23660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cp3 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.218 |
| Data reduction software | PROTEUM PLUS (PLUS) |
| Data scaling software | PROTEUM PLUS (PLUS) |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.900 | 3.030 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 16873 | |
| Completeness [%] | 90.5 | 95.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 50mM sodium cacodylate pH 6.0, 7.5% w/v PEG 4000, 1% w/v beta-D-glucopyranoside, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
