3NQW
A metazoan ortholog of SpoT hydrolyzes ppGpp and plays a role in starvation responses
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 6C1 |
| Synchrotron site | PAL/PLS |
| Beamline | 6C1 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2008-10-14 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.23985 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.000, 71.400, 49.900 |
| Unit cell angles | 90.00, 101.40, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.900 |
| Rwork | 0.213 |
| R-free | 0.28220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.190 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.1.4) |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 6.240 | 2.900 |
| Rmerge | 0.071 | 0.036 | 0.366 |
| Number of reflections | 7539 | ||
| <I/σ(I)> | 10.7 | ||
| Completeness [%] | 96.5 | 94 | 80.4 |
| Redundancy | 3.5 | 3.5 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 296 | 100mM sodium cacodylate (pH 6.5), 1.2-1.5M ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
