3NQJ
Crystal structure of (CENP-A/H4)2 heterotetramer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 193 |
| Detector technology | CCD |
| Collection date | 2010-04-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 62.869, 62.869, 159.512 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.100 |
| R-factor | 0.18249 |
| Rwork | 0.180 |
| R-free | 0.24141 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3nqu |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.329 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.450 | 2.210 |
| High resolution limit [Å] | 2.080 | 2.080 |
| Rmerge | 0.083 | 0.447 |
| Number of reflections | 11585 | |
| <I/σ(I)> | 23.39 | 4.3 |
| Completeness [%] | 97.6 | 87.7 |
| Redundancy | 8.22 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 0.17 M ammonium sulfate, 0.085 M sodium cacodylate (pH 6.5), 25.5% PEG-8000, 15% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
