3NQ4
30mer structure of Lumazine synthase from Salmonella typhimurium LT2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-04-10 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.542 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 174.430, 157.490, 202.788 |
| Unit cell angles | 90.00, 91.58, 90.00 |
Refinement procedure
| Resolution | 78.745 - 3.500 |
| R-factor | 0.2562 |
| Rwork | 0.255 |
| R-free | 0.29940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mk3 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.946 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 134.030 | 3.690 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.258 | 0.392 |
| Number of reflections | 45638 | |
| <I/σ(I)> | 3.2 | 2.2 |
| Completeness [%] | 66.5 | 70.2 |
| Redundancy | 2.2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.75 | 293 | 1.6M Ammonium Sulfate, 100mM Tris buffer, pH 7.75, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
