3NPZ
Prolactin Receptor (PRLR) Complexed with the Natural Hormone (PRL)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 273 |
| Detector technology | CCD |
| Collection date | 2007-09-02 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 92.174, 92.174, 216.072 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 84.782 - 3.350 |
| R-factor | 0.2366 |
| Rwork | 0.234 |
| R-free | 0.29250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ew3 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.318 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 84.820 | 3.530 |
| High resolution limit [Å] | 3.350 | 3.350 |
| Rmerge | 0.131 | 0.388 |
| Number of reflections | 13695 | |
| <I/σ(I)> | 22.7 | 7.6 |
| Completeness [%] | 97.8 | 97.5 |
| Redundancy | 11.7 | 12.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 291 | Sodium Citrate 100 mM, 14 % PEG 4000, 100 mM LiSO4, 12 % glycerol, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
