3NPA
Glycogen phosphorylase complexed with 2,5-dihydroxy-4-(beta-D-glucopyranosyl)-bromo-benzene
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX10.1 |
Synchrotron site | SRS |
Beamline | PX10.1 |
Temperature [K] | 298 |
Detector technology | CCD |
Collection date | 2005-05-24 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.488 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 128.702, 128.702, 116.439 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.969 |
R-factor | 0.19194 |
Rwork | 0.191 |
R-free | 0.21271 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 2prj |
RMSD bond length | 0.007 |
RMSD bond angle | 1.022 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.000 |
High resolution limit [Å] | 1.969 | 1.969 |
Rmerge | 0.065 | 0.298 |
Number of reflections | 64845 | |
<I/σ(I)> | 11.3 | 4.2 |
Completeness [%] | 93.9 | 90 |
Redundancy | 3.7 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | SMALL TUBES | 6.7 | 289 | 20 mg/mL protein in a buffer consisting of 10 mM BES, 0.5 mM EDTA, 3 mM DTT. T-state GPb crystals soaked with 20 mM inhibitor for 6 hrs, pH 6.7, SMALL TUBES, temperature 289K |