3NNA
Crystal Structure of CUGBP1 RRM1/2-RNA Complex
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-09-30 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97920, 0.97880, 0.96360 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 47.367, 70.061, 131.706 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.899 |
| R-factor | 0.20936 |
| Rwork | 0.207 |
| R-free | 0.24775 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.268 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.899 | 1.899 |
| Rmerge | 0.042 | 0.366 |
| Number of reflections | 33331 | |
| <I/σ(I)> | 36.3 | 3.7 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 0.2 M ammonium acetate, 45% MPD, 0.1 M Tris pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
