3NHN
Crystal structure of the SRC-family kinase HCK SH3-SH2-linker regulatory region
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E SUPERBRIGHT |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2009-07-07 |
Detector | RIGAKU SATURN 944 |
Wavelength(s) | 1.54 |
Spacegroup name | P 31 |
Unit cell lengths | 60.714, 60.714, 49.581 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.890 - 2.610 |
R-factor | 0.1198 |
Rwork | 0.119 |
R-free | 0.17640 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qcf |
RMSD bond length | 0.007 |
RMSD bond angle | 1.062 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.6_289) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.660 |
High resolution limit [Å] | 2.610 | 7.080 | 2.610 |
Rmerge | 0.056 | 0.039 | 0.278 |
Number of reflections | 6220 | ||
<I/σ(I)> | 33.6 | ||
Completeness [%] | 98.2 | 98.7 | 78.9 |
Redundancy | 3.4 | 3.8 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 25% (v/v) polyethylene glycol 3,350, 0.15 M KSCN, 0.05 M NaCl, and 1:100 dilution of Hck-SH3-SH2-linker crystal seeds (grown in 25% v/v polyethylene glycol 3,350, 0.1 M Tris pH 8.5, 0.2 M NaCl, VAPOR DIFFUSION, SITTING DROP, temperature 298K |