3NHE
High Resolution Structure (1.26A) of USP2a in Complex with Ubiquitin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-04-21 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 102.771, 53.961, 74.719 |
Unit cell angles | 90.00, 107.71, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.310 |
High resolution limit [Å] | 1.260 | 1.260 |
Rmerge | 0.066 | 0.258 |
Number of reflections | 103417 | |
<I/σ(I)> | 12.6 | |
Completeness [%] | 98.0 | 88.1 |
Redundancy | 3.5 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 295 | 5% PEG4000, 0.1M Hepes, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 295K |