3NHC
GYMLGS segment 127-132 from human prion with M129
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-19 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0048 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 18.150, 9.553, 45.163 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.580 - 1.570 |
| R-factor | 0.2246 |
| Rwork | 0.221 |
| R-free | 0.25280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.541 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (1.3.2) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 90.000 | 90.000 | 1.610 |
| High resolution limit [Å] | 1.550 | 3.340 | 1.550 |
| Rmerge | 0.186 | 0.137 | 0.398 |
| Number of reflections | 1163 | ||
| <I/σ(I)> | 9.4 | ||
| Completeness [%] | 90.9 | 96.8 | 78.2 |
| Redundancy | 5.4 | 4.8 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 0.2M Tris pH 7.0, vapor diffusion, hanging drop, temperature 298K |
