3NH0
Crystal structure of RNase T in complex with a non-preferred ssDNA (AAC)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-30 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.999 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 96.075, 106.360, 46.991 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.148 - 2.300 |
| R-factor | 0.1999 |
| Rwork | 0.196 |
| R-free | 0.24930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ngy |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.830 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | PHENIX ((phenix.refine: 1.6_289)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 22313 | |
| <I/σ(I)> | 23.82 | 6.2 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 5.8 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3 | 298 | 0.1M Citric acid pH 3.5, 14% PEG 1000, 10mM Spermidine, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
