3NGJ
Crystal structure of a putative deoxyribose-phosphate aldolase from Entamoeba histolytica
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-17 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.260, 70.780, 126.750 |
| Unit cell angles | 90.00, 92.46, 90.00 |
Refinement procedure
| Resolution | 47.380 - 1.700 |
| R-factor | 0.147 |
| Rwork | 0.146 |
| R-free | 0.17500 |
| Structure solution method | MR, MR |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.370 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.700 |
| Rmerge | 0.086 |
| Number of reflections | 96738 |
| <I/σ(I)> | 13.1 |
| Completeness [%] | 98.9 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 290 | MD PACT SCREEN CONDITION C12: 100MM HEPES, PH 7.0, 20% PEG 6000, 10MM ZNCL2. ENHIA.01205.A AT 42.7MG/ML, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
