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3NGB

Crystal structure of broadly and potently neutralizing antibody VRC01 in complex with HIV-1 gp120

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 22-ID
Synchrotron siteAPS
Beamline22-ID
Temperature [K]100
Detector technologyCCD
Collection date2009-10-31
DetectorMARMOSAIC 300 mm CCD
Wavelength(s)0.82656
Spacegroup nameP 1 21 1
Unit cell lengths108.630, 98.275, 205.256
Unit cell angles90.00, 99.68, 90.00
Refinement procedure
Resolution49.733 - 2.680
R-factor0.1997
Rwork0.197
R-free0.25630
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)3idx
RMSD bond length0.002
RMSD bond angle0.534
Data reduction softwareHKL-2000
Data scaling softwareHKL-2000
Phasing softwarePHASER
Refinement softwarePHENIX ((phenix.refine: 1.5_2))
Data quality characteristics
 Overall
Low resolution limit [Å]50.000
High resolution limit [Å]2.680
Number of reflections97150
<I/σ(I)>18.6
Completeness [%]94.8
Redundancy3.4
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP8.529310% PEG 4000, 9% Isopropanol, 0.1M Ammonium Sulfate, 0.1M Tris/Cl-, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K

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