3NBY
Crystal structure of the PKI NES-CRM1-RanGTP nuclear export complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-09-18 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.92000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.090, 223.726, 163.064 |
| Unit cell angles | 90.00, 100.63, 90.00 |
Refinement procedure
| Resolution | 38.632 - 3.420 |
| R-factor | 0.2607 |
| Rwork | 0.258 |
| R-free | 0.31450 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3gjx |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.545 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | CNS |
| Refinement software | PHENIX (1.6.1_357) |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 3.420 |
| Rmerge | 0.169 |
| Rmeas | 0.203 |
| Total number of observations | 169861 |
| Number of reflections | 57482 |
| <I/σ(I)> | 5.68 |
| Completeness [%] | 83.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.2 | 293 | 0.1 M Tris/HCl pH range 8.08-8.30, 10-14% (w/v) PEG 1000, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
