3NAU
Crystal structure of ZHX2 HD2 (zinc-fingers and homeoboxes protein 2, homeodomain 2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-11-28 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.976 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 96.710, 60.670, 27.710 |
Unit cell angles | 90.00, 95.34, 90.00 |
Refinement procedure
Resolution | 28.380 - 2.700 |
R-factor | 0.20664 |
Rwork | 0.203 |
R-free | 0.26684 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ecc |
RMSD bond length | 0.006 |
RMSD bond angle | 0.902 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.111 | 0.451 |
Number of reflections | 4459 | |
<I/σ(I)> | 15.4 | 3.1 |
Completeness [%] | 100.0 | |
Redundancy | 5.6 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 294 | 20% PEG 6000, 0.2M LiCl, HEPES pH7.0, VAPOR DIFFUSION, SITTING DROP, temperature 294K |