3NAQ

Apo-form of NAD-dependent formate dehydrogenase from higher-plant Arabidopsis thaliana

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X13
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X13
Temperature [K]	100
Detector technology	CCD
Detector	MAR CCD 165 mm
Wavelength(s)	0.81
Spacegroup name	P 21 21 21
Unit cell lengths	70.330, 106.230, 106.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	75.160 - 1.700
R-factor	0.16894
Rwork	0.167
R-free	0.19718
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.017
RMSD bond angle	1.589
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	75.160	1.800
High resolution limit [Å]	1.700	1.700
Rmerge	0.046	0.323
Number of reflections	87821
<I/σ(I)>	31.25	5.36
Completeness [%]	95.9	90.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	Protein solution (2ul): 6 mg/ml FDH, 0.1M Na2HPO4, pH 7.0, 10 mM EDTA. Reservoir solution (2ul): 0.1M Bis-Tris, pH 6.0, 2.1M Ammonium Sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 293K