3NAM
SR Ca(2+)-ATPase in the HnE2 state complexed with the Thapsigargin derivative dOTg
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-25 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.2548 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 71.545, 71.545, 591.227 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.100 |
| Rwork | 0.239 |
| R-free | 0.28000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2c8k |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Number of reflections | 26884 | |
| <I/σ(I)> | 11.18 | 1.9 |
| Completeness [%] | 90.6 | 75.4 |
| Redundancy | 5.2 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 298 | 8% w/v PEG 2000 MME, 4% v/v glycerol, 0.05M NaCl, 4% v/v 2-methyl-2,4-pentanediol, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
