3N9O

ceKDM7A from C.elegans, complex with H3K4me3 peptide, H3K9me2 peptide and NOG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2009-05-06
Detector	RAYONIX MX-225
Wavelength(s)	1.2828
Spacegroup name	P 21 21 21
Unit cell lengths	69.090, 87.379, 102.727
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.280 - 2.309
R-factor	0.2014
Rwork	0.199
R-free	0.24210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3n9m
RMSD bond length	0.007
RMSD bond angle	1.016
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.300	2.300
Number of reflections	27497
Completeness [%]	98.4	87.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277	16% PEG 3350, 0.2M Sodium Fluoride, 0.1M Bis-Tris or HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K